Abilities

1.3 Gbp) were processed to remove SMRT bell adapters, short and low-quality reads (< 80% accuracy) using SMRT Analysis version 2.3 ( A total of 155,828 filtered reads (average length, 8.9 Kb) were used for de novo assembly using Celera Assembler , with self-correction of the PacBio reads. A total of 1091 Mb paired-end Illumina reads screened from a 500-bp genomic library were mapped using SOAP to the bacterial plasmid database for tracing the presence of any plasmid and filling the gaps. The de novo assembly resulted in five contigs, representing the K. michiganensis BD177 complete genome of 6,812,698 bp with GC content % in a single chromosome and four plasmids. The annotated genome contains 6714 genes, 25rRNA genes, and 86 tRNA genes (Table 1).

16S rRNA phylogenetic study and you can phenotypic of isolated strain BD177

Maximum parsimony dependent and you can maximum probability phylogenetic trees using the succession you to definitely encodes with the 16S rRNA gene showed that the fresh new closest types so you can BD177 are K. michiganensis and you will K. oxytica (Extra file step 1: Fig. S1, Most document dos: Dining table S1). Evolutionary divergence distance out-of 16S rRNA gene sequence anywhere between BD177 and K. michiganensis E718 are minimum worthy of (BD177 versus K. michiganensis E718: 0.0079) certainly every sequences. Biochemical testing was basically performed on BD177 by API20E to confirm their condition regarding K. oxytoca and you will K. michiganensis. Biochemical features off K. oxytoca ATCC13182(T)(=NCTC13727) and you may K. michiganensis W14(T)(= DSM2544) was in fact taken from BacDive Webservices pared that have K. oxytoca ATCC13182(T) and you will K. michiganensis W14(T), BD177 is the same that have W14 on ?-galactosidase chemical confident and you can urease https://datingranking.net/tr/farmers-dating-site-inceleme negative, exact same having ATCC13182(T) when you look at the arginine dihydrolase confident. (more…)